First rule of writing is to write what you're passionate about. It's not just "write what you know" any more. It's about writing what you want to know more about.
Now, that you've picked your topic, you've discovered which is the best POV for your story, take a look at your audience. Perception of the story can be different depending on age, gender, culture or basically any experience.
As a writer, we try to determine what is the demographic of those who will read or story, but that can't be the only driven factor. We must know as we write that we can't please everyone. Take your story, give it to four people to read, of different ages, and different genders, and wait for the feedback. Each one will be different (and you want it that way).
I recently sent a story of mine to different readers, and the feedback was different. I learned from both feedback and was able to incorporate into the story what I knew made sense and make it more accurate and appealing, but then I also knew what I should leave alone and keep as part of the core of the story.
Perceptions:
Character perceptions. Characters may be judged or assumed differently from different readers based on experience, values or other aspects tat make them different. Some may see a character who is sexually active as sexually independent and strong, others may see her as a ... well, (insert inappropriate word here).
Even when reading, readers judge and assume things about a character, even if the writer intended something different.
Be aware of perceptions, but in any case, be true to your writing and your characters. They rely on you for their life and adventures.